Blood Analyses

Material and Methods

Material

Six cancer patients suffering from pickle cell carcinoma of esophagus, malignant melanoma, liver cancer, malignant lymphoma, bladder cancer and breast cancer. Six control patients with hepatitis (14a), chr. tonsillitis (41a), M. Parkinson (78a), healthy (12a), healthy (14a), healthy (20a). For the diagnoses and the material I have to thank R.Pekar.

The MF-procedure

(M = maceration, F = filtration)

 

  1. 10 ml with heparin anticoagulated peripheral blood
  2. freezing at – 20°C
  3. warming up to 36°C
  4. 1ml suspended in 7,5% KOH – solution and macer ation 7 hours incubated at 36°C
  5. filtration through 3µm, 0,8µm, 0,4µm and 0,1µm polycarbonate – filter
  6. rinsing in ster. Ringer solution
  7. fixation in 4,5% glutaraldehyde buffered with 0,1 M Na – cacodylate for 2 h
  8. rinsing in 0,1 M Na – cacodylate
  9. dehydration in ethanol 35%, 70%, 80%, 95%, 2X 100%
  10. aceton and critical point drying for SEM
  11. or propylenoxide for TEM embedding procedure (Luft), ultrathin sections were contrasted with uranylacetate/leadcitrate
  12. Counting of 0,25 particles and calculating their number up to 1µl blood.

Results

Between the groups of cancer patients and the control group the following differences became evident:  In the blood of a cancer patient 21.917 +/- 913 particles per µl were found (17.444 – 26.389; n=6). In the control  group 1220 +/- 478 particles per µl blood were counted (0 – 3358 ; n=6). After the t-test a significant difference - admitting an error probability of 5% - was found. The difference becomes clear in fig.1 compared with fig. 2a and 2b. Also nano – colonies of these particles ( fig. 3a) like a corncob could be detected. In fig. 3b one can see a nano – colony with small crystales between the particles.

 

Fig.1 Particles of a healthy person (12a, male) in SEM, magnification 6250 : 1

 

Fig. 2a Particles (Basoplasma sanguineum) of a cancer-patient (liver-cancer, male) in SEM, magnification 6250 : 1. ( higher resolution)

 

Fig. 2b Particles (Basoplasma sanguineum) of a cancer-patient (breast-cancer, 44a) in SEM, magnification 6250 : 1

 

Fig. 3a Nano-colony of particles (Basoplasma sanguineum), prickle cell carcinoma of esophagus in SEM, magnification  6250 : 1. (higher resolution)

 

Fig. 3b Nano-colony of particles (Basoplasma sanguineum), prickle cell carcinoma of esophagus in SEM, magnification  6250 : 1. (higher resolution)

 

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